RFX3 modulation of FOXJ1 regulation of cilia genes in the human airway epithelium.

ABSTRACT:

Background

Ciliated cells play a central role in cleansing the airways of inhaled contaminants. They are derived from basal cells that include the airway stem/progenitor cells. In animal models, the transcription factor FOXJ1 has been shown to induce differentiation to the ciliated cell lineage, and the RFX transcription factor-family has been shown to be necessary for, but not sufficient to induce, correct cilia development.

Methods

To test the hypothesis that FOXJ1 and RFX3 cooperatively induce expression of ciliated genes in the differentiation process of basal progenitor cells toward a ciliated cell linage in the human airway epithelium, primary human airway basal cells were assessed under conditions of in vitro differentiation induced by plasmid-mediated gene transfer o

SUBMITTER: Didon L 

PROVIDER: S-EPMC3710277 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature