Noninvasive monitoring of oxidative stress in transplanted mesenchymal stromal cells.

ABSTRACT:

Objectives

The goal of this study was to validate a pathway-specific reporter gene that could be used to noninvasively image the oxidative status of progenitor cells.

Background

In cell therapy studies, reporter gene imaging plays a valuable role in the assessment of cell fate in living subjects. After myocardial injury, noxious stimuli in the host tissue confer oxidative stress to transplanted cells that may influence their survival and reparative function.

Methods

Rat mesenchymal stromal cells (MSCs) were studied for phenotypic evidence of increased oxidative stress under in vitro stress. On the basis of their up-regulation of the pro-oxidant enzyme p67(phox) subunit of nicotinamide adenine dinucleotide phosphate (NAD[P]H oxidase p67(phox)), an oxidative stress sens

SUBMITTER: Psaltis PJ 

PROVIDER: S-EPMC3710523 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature