Mechanisms of in vivo binding site selection of the hematopoietic master transcription factor PU.1.

ABSTRACT: The transcription factor PU.1 is crucial for the development of many hematopoietic lineages and its binding patterns significantly change during differentiation processes. However, the 'rules' for binding or not-binding of potential binding sites are only partially understood. To unveil basic characteristics of PU.1 binding site selection in different cell types, we studied the binding properties of PU.1 during human macrophage differentiation. Using in vivo and in vitro binding assays, as well as computational prediction, we show that PU.1 selects its binding sites primarily based on sequence affinity, which results in the frequent autonomous binding of high affinity sites in DNase I inaccessible regions (25-45% of all occupied sites). Increasing PU.1 concentrations and the availability o

SUBMITTER: Pham TH 

PROVIDER: S-EPMC3711439 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature