Live cell imaging reveals marked variability in myoblast proliferation and fate.

ABSTRACT:

Background

During the process of muscle regeneration, activated stem cells termed satellite cells proliferate, and then differentiate to form new myofibers that restore the injured area. Yet not all satellite cells contribute to muscle repair. Some continue to proliferate, others die, and others become quiescent and are available for regeneration following subsequent injury. The mechanisms that regulate the adoption of different cell fates in a muscle cell precursor population remain unclear.

Methods

We have used live cell imaging and lineage tracing to study cell fate in the C2 myoblast line.

Results

Analyzing the behavior of individual myoblasts revealed marked variability in both cell cycle duration and viability, but similarities between cells derived from the sam

SUBMITTER: Gross SM 

PROVIDER: S-EPMC3712004 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature