Architectural protein subclasses shape 3D organization of genomes during lineage commitment.

ABSTRACT: Understanding the topological configurations of chromatin may reveal valuable insights into how the genome and epigenome act in concert to control cell fate during development. Here, we generate high-resolution architecture maps across seven genomic loci in embryonic stem cells and neural progenitor cells. We observe a hierarchy of 3D interactions that undergo marked reorganization at the submegabase scale during differentiation. Distinct combinations of CCCTC-binding factor (CTCF), Mediator, and cohesin show widespread enrichment in chromatin interactions at different length scales. CTCF/cohesin anchor long-range constitutive interactions that might form the topological basis for invariant subdomains. Conversely, Mediator/cohesin bridge short-range enhancer-promoter interactions within an

SUBMITTER: Phillips-Cremins JE 

PROVIDER: S-EPMC3712340 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature