Dynamic localization of G-actin during membrane protrusion in neuronal motility.

ABSTRACT:

Background

Actin-based cell motility is fundamental for development, function, and malignant events in eukaryotic organisms. During neural development, axonal growth cones depend on rapid assembly and disassembly of actin filaments (F-actin) for their guided extension to specific targets for wiring. Monomeric globular actin (G-actin) is the building block for F-actin but is not considered to play a direct role in spatiotemporal control of actin dynamics in cell motility.

Results

Here we report that a pool of G-actin dynamically localizes to the leading edge of growth cones and neuroblastoma cells to spatially elevate the G-/F-actin ratio that drives membrane protrusion and cell movement. Loss of G-actin localization leads to the cessation and retraction of membrane protrusio

SUBMITTER: Lee CW 

PROVIDER: S-EPMC3712510 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature