A dual-color genetically engineered mouse model for multispectral imaging of the pancreatic microenvironment.

ABSTRACT:

Objectives

To develop a mouse model for multispectral fluorescence imaging of the pancreas and pancreatic microenvironment.

Methods

Cre/loxP technology was used to develop this model. We crossed mT/mG indicator mice, engineered to constitutively express a conditional tdTomato transgene that converts to green fluorescent protein (GFP) expression after exposure to Cre recombinase, with Pdx1-Cre transgenic mice. To characterize this model for studies of pancreas biology, we performed bright light and fluorescence imaging of body cavities and intact organs and confocal microscopy of pancreata from offspring of Pdx1-Cre and mT/mG crosses.

Results

Pdx1-Cre-mT/mG mice demonstrated bright GFP expression within the pancreas and duodenum and intense tdTomato expression in all o

SUBMITTER: Snyder CS 

PROVIDER: S-EPMC3713119 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature