A single DH gene segment is sufficient for the establishment of an asthma phenotype in a murine model of allergic airway inflammation.

ABSTRACT:

Background

We have previously shown that the allergic sensitization to ovalbumin does not represent a superantigen-like immune response. In gene-targeted mice (ΔD-iD) with a single modified Diversity gene segment (D(H)) of the immunoglobulin heavy chain, enriched for charged amino acids, the asthma phenotype in a murine model was markedly alleviated compared to wild-type animals.

Objective

We now sought to determine whether the confinement to a single D(H) gene segment alone leads to a reduced allergic phenotype.

Methods

We examined another gene-targeted mouse strain (ΔD-DFL) with a single D(H) gene segment which encodes for neutral amino acids, thus reflecting the preferential repertoire in wild-type mice. Mice were sensitized intraperitoneally to ovalbumin.

Resul

SUBMITTER: Kerzel S 

PROVIDER: S-EPMC3713642 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature