Extensive modulation of the transcription factor transcriptome during somatic embryogenesis in Arabidopsis thaliana.

ABSTRACT: Molecular mechanisms controlling plant totipotency are largely unknown and studies on somatic embryogenesis (SE), the process through which already differentiated cells reverse their developmental program and become embryogenic, provide a unique means for deciphering molecular mechanisms controlling developmental plasticity of somatic cells. Among various factors essential for embryogenic transition of somatic cells transcription factors (TFs), crucial regulators of genetic programs, are believed to play a central role. Herein, we used quantitative real-time polymerase chain reaction (qRT-PCR) to identify TF genes affected during SE induced by in vitro culture in Arabidopsis thaliana. Expression profiles of 1,880 TFs were evaluated in the highly embryogenic Col-0 accession and the non-embr

SUBMITTER: Gliwicka M 

PROVIDER: S-EPMC3714258 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature