Epidermal growth factor: the driving force in initiation of RPE cell proliferation.

ABSTRACT:

Background

To analyze whether epidermal growth factor (EGF) exerts regulatory effects on proliferation and differentiation in ARPE19 cells after different incubation periods (24 vs. 48 h) for obtaining ideal conditions for feasible rejuvenation and autologous transplantation of retinal pigment epithelial cells (RPE cells).

Methods

To evaluate gene expression patterns of RPE-specific differentiation and proliferation markers as well as transcriptional and translational changes of beta-catenin (ß-catenin)-signaling markers by fluorescence activated cell sorting (FACS) and reverse transcription - polymerase chain reaction (RT-PCR) after 24 h of EGF treatment.

Results

After 24 h of EGF treatment, a significant decrease of retinal pigment epithelium-specific protein 65 (RP

SUBMITTER: Steindl-Kuscher K 

PROVIDER: S-EPMC3714846 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature