The human TUT1 nucleotidyl transferase as a global regulator of microRNA abundance.

ABSTRACT: Post-transcriptional modifications of miRNAs with 3' non-templated nucleotide additions (NTA) are a common phenomenon, and for a handful of miRNAs the additions have been demonstrated to modulate miRNA stability. However, it is unknown for the vast majority of miRNAs whether nucleotide additions are associated with changes in miRNA expression levels. We previously showed that miRNA 3' additions are regulated by multiple nucleotidyl transferase enzymes. Here we examine the changes in abundance of miRNAs that exhibit altered 3' NTA following the suppression of a panel of nucleotidyl transferases in cancer cell lines. Among the miRNAs examined, those with increased 3' additions showed a significant decrease in abundance. More specifically, miRNAs that gained a 3' uridine were associated with

SUBMITTER: Knouf EC 

PROVIDER: S-EPMC3715485 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature