A novel single virus infection system reveals that influenza virus preferentially infects cells in g1 phase.

ABSTRACT:

Background

Influenza virus attaches to sialic acid residues on the surface of host cells via the hemagglutinin (HA), a glycoprotein expressed on the viral envelope, and enters into the cytoplasm by receptor-mediated endocytosis. The viral genome is released and transported in to the nucleus, where transcription and replication take place. However, cellular factors affecting the influenza virus infection such as the cell cycle remain uncharacterized.

Methods/results

To resolve the influence of cell cycle on influenza virus infection, we performed a single-virus infection analysis using optical tweezers. Using this newly developed single-virus infection system, the fluorescence-labeled influenza virus was trapped on a microchip using a laser (1064 nm) at 0.6 W, transported, an

SUBMITTER: Ueda R 

PROVIDER: S-EPMC3715512 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature