Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes.

ABSTRACT: The regulation of antibody reporting intensities is critical to various in situ fluorescence-imaging analyses. Although such control is often necessary to visualize sparse molecular targets, the ability to tune marker intensities is also essential for highly multiplexed imaging strategies in which marker reporting levels must be tuned both to optimize dynamic detection ranges and to minimize crosstalk between different signals. Existing chemical amplification approaches generally lack such control. Here, we demonstrate that linear and branched DNA complexes can be designed to function as interchangeable building blocks that can be assembled into organized, fluorescence-reporting complexes. We show that the ability to program DNA-strand-displacement reactions between these complexes offers

SUBMITTER: Zimak J 

PROVIDER: S-EPMC3715551 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature