Microbiological diagnosis of severe diarrhea in kidney transplant recipients by use of multiplex PCR assays.

ABSTRACT: Diarrhea is a frequent complication after kidney transplantation, ascribed to adverse effects of the immunosuppressive therapy in case of negative microbiological examination of the stools. The aim of this study was to improve the microbiological diagnosis by implementing molecular tests. Fifty-four severe diarrhea events that occurred in 49 adult kidney transplant recipients from September 2010 to November 2011 were investigated. One or several enteric pathogens were detected in 13 (23%) stool samples using classical microbiological methods versus 39 (72%) for the seven commercially available multiplex PCR assays used retrospectively (P = 0.006). Interestingly, molecular diagnosis identified 15 multiple infections compared to none using classical techniques. The primary pathogens detected

SUBMITTER: Coste JF 

PROVIDER: S-EPMC3716061 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature