Regioselective covalent immobilization of recombinant antibody-binding proteins A, G, and L for construction of antibody arrays.

ABSTRACT: Immobilized antibodies are useful for the detection of antigens in highly sensitive microarray diagnostic applications. Arrays with the antibodies attached regioselectively in a uniform orientation are typically more sensitive than those with random orientations. Direct regioselective immobilization of antibodies on a solid support typically requires a modified form of the protein. We now report a general approach for the regioselective attachment of antibodies to a surface using truncated forms of antibody-binding proteins A, G, and L that retain the structural motifs required for antibody binding. The recombinant proteins have a C-terminal CVIX protein farnesyltransferase recognition motif that allows us to append a bioorthogonal azide or alkyne moiety and use the Cu(I)-catalyzed Huisgen

SUBMITTER: Seo JS 

PROVIDER: S-EPMC3716362 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature