Iterative in situ click chemistry creates antibody-like protein-capture agents.

ABSTRACT: Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.

SUBMITTER: Agnew HD 

PROVIDER: S-EPMC3716464 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature