Lipopolysaccharide induces the expression of an autocrine prolactin loop enhancing inflammatory response in monocytes.

ABSTRACT:

Background

Prolactin from pituitary gland helps maintain homeostasis but it is also released in immune cells where its function is not completely understood. Pleiotropic functions of prolactin (PRL) might be mediated by different isoforms of its receptor (PRLr).

Methods

The aim of this study was to investigate the relationship between the eventual synthesis of PRL and PRLr isoforms with the inflammatory response in monocytes. We used THP-1 and monocytes isolated from healthy subjects stimulated with lipopolysaccharide (LPS). Western blot, real time PCR and immunocytochemistry were performed to identify both molecules. The bioactivity of the PRL was assessed using a bioassay and ELISA to detect pro inflammatory cytokines.

Results

PRLr mRNA and PRL mRNA were synthesized

SUBMITTER: Lopez-Rincon G 

PROVIDER: S-EPMC3716533 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature