Digital genotyping of sorghum - a diverse plant species with a large repeat-rich genome.

ABSTRACT:

Background

Rapid acquisition of accurate genotyping information is essential for all genetic marker-based studies. For species with relatively small genomes, complete genome resequencing is a feasible approach for genotyping; however, for species with large and highly repetitive genomes, the acquisition of whole genome sequences for the purpose of genotyping is still relatively inefficient and too expensive to be carried out on a high-throughput basis. Sorghum bicolor is a C4 grass with a sequenced genome size of ~730 Mb, of which ~80% is highly repetitive. We have developed a restriction enzyme targeted genome resequencing method for genetic analysis, termed Digital Genotyping (DG), to be applied to sorghum and other grass species with large repeat-rich genomes.

Results

DG

SUBMITTER: Morishige DT 

PROVIDER: S-EPMC3716661 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature