Identification of thyroid hormone receptor binding sites in developing mouse cerebellum.

ABSTRACT:

Background

Thyroid hormones play an essential role in early vertebrate development as well as other key processes. One of its modes of action is to bind to the thyroid hormone receptor (TR) which, in turn, binds to thyroid response elements (TREs) in promoter regions of target genes. The sequence motif for TREs remains largely undefined as does the precise chromosomal location of the TR binding sites. A chromatin immunoprecipitation on microarray (ChIP-chip) experiment was conducted using mouse cerebellum post natal day (PND) 4 and PND15 for the thyroid hormone receptor (TR) beta 1 to map its binding sites on over 5000 gene promoter regions. We have performed a detailed computational analysis of these data.

Results

By analysing a recent spike-in study, the optimal normalizat

SUBMITTER: Gagne R 

PROVIDER: S-EPMC3716714 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature