Sulfur-regulated control of the met-2⁺ gene of Neurospora crassa encoding cystathionine β-lyase.

ABSTRACT:

Background

Cystathionine β-lyase performs an essential role in the transsulfuration pathway by its primary reaction of forming homocysteine from cystathionine. Understanding how the Neurospora crassa met-2⁺ gene, which encodes cystathionine β-lyase, is regulated is important in determining the basis of the cellular control of transsulfuration. The aim of this study was to determine the nature of a potential regulatory connection of met-2⁺ to the Neurospora sulfur regulatory network.

Findings

The cystathionine β-lyase (met-2⁺) gene was cloned by the identification of a cosmid genomic clone capable of transforming a met-2 mutant to methionine prototrophy and subsequently characterized. The gene contains a single intron and encodes a protein of 457 amino acids with conserved re

SUBMITTER: Reveal BS 

PROVIDER: S-EPMC3716945 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature