An enzyme linked immunosorbent assay (ELISA) for the determination of the human haptoglobin phenotype.

ABSTRACT:

Background

Haptoglobin (Hp) is an abundant serum protein which binds extracorpuscular hemoglobin (Hb). Two alleles exist in humans for the Hp gene, denoted 1 and 2. Diabetic individuals with the Hp 2-2 genotype are at increased risk of developing vascular complications including heart attack, stroke, and kidney disease. Recent evidence shows that treatment with vitamin E can reduce the risk of diabetic vascular complications by as much as 50% in Hp 2-2 individuals. We sought to develop a rapid and accurate test for Hp phenotype (which is 100% concordant with the three major Hp genotypes) to facilitate widespread diagnostic testing as well as prospective clinical trials.

Methods

A monoclonal antibody raised against human Hp was shown to distinguish between the three Hp phenot

SUBMITTER: Levy NS 

PROVIDER: S-EPMC3717392 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature