Mapping gene clusters within arrayed metagenomic libraries to expand the structural diversity of biomedically relevant natural products.

ABSTRACT: Complex microbial ecosystems contain large reservoirs of unexplored biosynthetic diversity. Here we provide an experimental framework and data analysis tool to facilitate the targeted discovery of natural-product biosynthetic gene clusters from the environment. Multiplex sequencing of barcoded PCR amplicons is followed by sequence similarity directed data parsing to identify sequences bearing close resemblance to biosynthetically or biomedically interesting gene clusters. Amplicons are then mapped onto arrayed metagenomic libraries to guide the recovery of targeted gene clusters. When applied to adenylation- and ketosynthase-domain amplicons derived from saturating soil DNA libraries, our analysis pipeline led to the recovery of biosynthetic clusters predicted to encode for previously unch

SUBMITTER: Owen JG 

PROVIDER: S-EPMC3718090 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature