Combined small RNA and degradome sequencing reveals novel miRNAs and their targets in response to low nitrate availability in maize.

ABSTRACT:

Background and aims

MicroRNAs (miRNAs) play an important role in the responses and adaptation of plants to many stresses including low nitrogen (LN). Characterizing relevant miRNAs will improve our understanding of nitrogen (N) use efficiency and LN tolerance and thus contribute to sustainable maize production. The objective of this study was to identify novel and known miRNAs and their targets involved in the response and adaptation of maize (Zea mays) to LN stress.

Methods

MiRNAs and their targets were identified by combined analysis of deep sequencing of small RNA and degradome libraries. The identity of target genes was confirmed by gene-specific RNA ligase-mediated rapid amplification of 5' cDNA ends (RLM-RACE) and by quantitative expression analysis.

Key results

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC3718221 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature