Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation.

ABSTRACT:

Aim

To investigate large tumor suppressor 1 (LATS1) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).

Methods

RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis.

Results

Decreased LATS1 e

SUBMITTER: Wierzbicki PM 

PROVIDER: S-EPMC3718905 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature