Protein analysis by time-resolved measurements with an electro-switchable DNA chip.

ABSTRACT: Measurements in stationary or mobile phases are fundamental principles in protein analysis. Although the immobilization of molecules on solid supports allows for the parallel analysis of interactions, properties like size or shape are usually inferred from the molecular mobility under the influence of external forces. However, as these principles are mutually exclusive, a comprehensive characterization of proteins usually involves a multi-step workflow. Here we show how these measurement modalities can be reconciled by tethering proteins to a surface via dynamically actuated nanolevers. Short DNA strands, which are switched by alternating electric fields, are employed as capture probes to bind target proteins. By swaying the proteins over nanometre amplitudes and comparing their motional d

SUBMITTER: Langer A 

PROVIDER: S-EPMC3719012 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature