Functional screening of metagenome and genome libraries for detection of novel flavonoid-modifying enzymes.

ABSTRACT: The functional detection of novel enzymes other than hydrolases from metagenomes is limited since only a very few reliable screening procedures are available that allow the rapid screening of large clone libraries. For the discovery of flavonoid-modifying enzymes in genome and metagenome clone libraries, we have developed a new screening system based on high-performance thin-layer chromatography (HPTLC). This metagenome extract thin-layer chromatography analysis (META) allows the rapid detection of glycosyltransferase (GT) and also other flavonoid-modifying activities. The developed screening method is highly sensitive, and an amount of 4 ng of modified flavonoid molecules can be detected. This novel technology was validated against a control library of 1,920 fosmid clones generated from a

SUBMITTER: Rabausch U 

PROVIDER: S-EPMC3719526 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature