Adenovirus E1A oncogene induces rereplication of cellular DNA and alters DNA replication dynamics.

ABSTRACT: The oncogenic property of the adenovirus (Ad) transforming E1A protein is linked to its capacity to induce cellular DNA synthesis which occurs as a result of its interaction with several host proteins, including pRb and p300/CBP. While the proteins that contribute to the forced induction of cellular DNA synthesis have been intensively studied, the nature of the cellular DNA replication that is induced by E1A in quiescent cells is not well understood. Here we show that E1A expression in quiescent cells leads to massive cellular DNA rereplication in late S phase. Using a single-molecule DNA fiber assay, we studied the cellular DNA replication dynamics in E1A-expressing cells. Our studies show that the DNA replication pattern is dramatically altered in E1A-expressing cells, with increased rep

SUBMITTER: Singhal G 

PROVIDER: S-EPMC3719816 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature