A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.

ABSTRACT: Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe(III)-to-Fe(II) reduction potential and substantially improves NAD(P)H-driven activity.

SUBMITTER: Coelho PS 

PROVIDER: S-EPMC3720782 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature