A framework for analysis of abortive colony size distributions using a model of branching processes in irradiated normal human fibroblasts.

ABSTRACT:

Background

Clonogenicity gives important information about the cellular reproductive potential following ionizing irradiation, but an abortive colony that fails to continue to grow remains poorly characterized. It was recently reported that the fraction of abortive colonies increases with increasing dose. Thus, we set out to investigate the production kinetics of abortive colonies using a model of branching processes.

Methodology/principal findings

We firstly plotted the experimentally determined colony size distribution of abortive colonies in irradiated normal human fibroblasts, and found the linear relationship on the log-linear or log-log plot. By applying the simple model of branching processes to the linear relationship, we found the persistent reproductive cell death

SUBMITTER: Sakashita T 

PROVIDER: S-EPMC3720916 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature