Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer.

ABSTRACT:

Background

MicroRNAs are noncoding regulatory RNAs strongly implicated in carcinogenesis, cell survival, and chemosensitivity. Here, microRNAs associated with chemoresistance in ovarian carcinoma, the most lethal of gynaecological malignancies, were identified and their functional effects in chemoresistant ovarian cancer cells were assessed.

Methods

MicroRNA expression in paclitaxel (PTX)-resistant SKpac sublines was compared with that of the PTX-sensitive, parental SKOV3 ovarian cancer cell line using microarray and qRT-PCR. The function of differentially expressed microRNAs in chemoresistant ovarian cancer was further evaluated by apoptosis, cell proliferation, and migration assays.

Results

Upregulation of miR-106a and downregulation of miR-591 were associated with

SUBMITTER: Huh JH 

PROVIDER: S-EPMC3721386 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature