Genetic and cellular evidence of decreased inflammation associated with reduced incidence of posttraumatic arthritis in MRL/MpJ mice.

ABSTRACT:

Objective

To examine the relationship between inflammation and posttraumatic arthritis (PTA) in a murine intraarticular fracture model.

Methods

Male C57BL/6 and MRL/MpJ "superhealer" mice received tibial plateau fractures using a previously established method. Mice were killed on day 0 (within 4 hours of fracture) and days 1, 3, 5, 7, 28, and 56 after fracture. Synovial tissue samples, obtained prior to fracture and on days 0, 1, 3, 5, and 7 after fracture, were examined by reverse transcription-polymerase chain reaction for gene expression of proinflammatory cytokines and chemokines. Synovial fluid and serum samples were collected to measure cytokine concentrations, using enzyme-linked immunosorbent assay. Whole joints were examined histologically for the extent of synoviti

SUBMITTER: Lewis JS 

PROVIDER: S-EPMC3721663 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature