Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores.

ABSTRACT: Kinetochores are the macromolecular complexes that interact with microtubules to mediate chromosome segregation. Accurate segregation requires that kinetochores make bioriented attachments to microtubules from opposite poles. Attachments between kinetochores and microtubules are monitored by the spindle checkpoint, a surveillance system that prevents anaphase until every pair of chromosomes makes proper bioriented attachments. Checkpoint activity is correlated with the recruitment of checkpoint proteins to the kinetochore. Mps1 is a conserved protein kinase that regulates segregation and the spindle checkpoint, but few of the targets that mediate its functions have been identified. Here, we show that Mps1 is the major kinase activity that copurifies with budding yeast kinetochore particles

SUBMITTER: London N 

PROVIDER: S-EPMC3723133 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature