Transcriptomic profiles of high and low antibody responders to smallpox vaccine.

ABSTRACT: Despite its eradication over 30 years ago, smallpox (as well as other orthopox viruses) remains a pathogen of interest both in terms of biodefense and for its use as a vector for vaccines and immunotherapies. Here we describe the application of mRNA-Seq transcriptome profiling to understanding immune responses in smallpox vaccine recipients. Contrary to other studies examining gene expression in virally infected cell lines, we utilized a mixed population of peripheral blood mononuclear cells in order to capture the essential intercellular interactions that occur in vivo, and would otherwise be lost, using single cell lines or isolated primary cell subsets. In this mixed cell population we were able to detect expression of all annotated vaccinia genes. On the host side, a number of genes en

SUBMITTER: Kennedy RB 

PROVIDER: S-EPMC3723701 | biostudies-literature | 2013 Jul-Aug

REPOSITORIES: biostudies-literature