Metabolic analyses elucidate non-trivial gene targets for amplifying dihydroartemisinic acid production in yeast.

ABSTRACT: Synthetic biology enables metabolic engineering of industrial microbes to synthesize value-added molecules. In this, a major challenge is the efficient redirection of carbon to the desired metabolic pathways. Pinpointing strategies toward this goal requires an in-depth investigation of the metabolic landscape of the organism, particularly primary metabolism, to identify precursor and cofactor availability for the target compound. The potent antimalarial therapeutic artemisinin and its precursors are promising candidate molecules for production in microbial hosts. Recent advances have demonstrated the production of artemisinin precursors in engineered yeast strains as an alternative to extraction from plants. We report the application of in silico and in vivo metabolic pathway analyses to i

SUBMITTER: Misra A 

PROVIDER: S-EPMC3724057 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature