Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus.

ABSTRACT:

Background

Daptomycin is an important antibiotic against infections caused by drug-resistant pathogens. Its production critically depends on the addition of decanoic acid during fermentation. Unfortunately, decanoic acid (>2.5 mM) is toxic to daptomycin producer, Streptomyces roseosporus.

Results

To understand the mechanism underlying decanoic tolerance or toxicity, the responses of S. roseosporus was determined by a combination of phospholipid fatty acid analysis, reactive oxygen species (ROS) measurement and RNA sequencing. Assays using fluorescent dyes indicated a sharp increase in reactive oxygen species during decanoic acid stress; fatty acid analysis revealed a marked increase in the composition of branched-chain fatty acids by approximately 10%, with a corresponding d

SUBMITTER: Liao G 

PROVIDER: S-EPMC3724488 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature