Identification of the likely translational start of Mycobacterium tuberculosis GyrB.

ABSTRACT:

Background

Bacterial DNA gyrase is a validated target for antibacterial chemotherapy. It consists of two subunits, GyrA and GyrB, which form an A₂B₂ complex in the active enzyme. Sequence alignment of Mycobacterium tuberculosis GyrB with other bacterial GyrBs predicts the presence of 40 potential additional amino acids at the GyrB N-terminus. There are discrepancies between the M. tuberculosis GyrB sequences retrieved from different databases, including sequences annotated with or without the additional 40 amino acids. This has resulted in differences in the GyrB sequence numbering that has led to the reporting of previously known fluoroquinolone-resistance mutations as novel mutations.

Findings

We have expressed M. tuberculosis GyrB with and without the extra 40 amino acids

SUBMITTER: Karkare S 

PROVIDER: S-EPMC3724585 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature