Transcription coactivators p300 and CBP are necessary for photoreceptor-specific chromatin organization and gene expression.

ABSTRACT: Rod and cone photoreceptor neurons in the mammalian retina possess specialized cellular architecture and functional features for converting light to a neuronal signal. Establishing and maintaining these characteristics requires appropriate expression of a specific set of genes, which is tightly regulated by a network of photoreceptor transcription factors centered on the cone-rod homeobox protein CRX. CRX recruits transcription coactivators p300 and CBP to acetylate promoter-bound histones and activate transcription of target genes. To further elucidate the role of these two coactivators, we conditionally knocked out Ep300 and/or CrebBP in differentiating rods or cones, using opsin-driven Cre recombinase. Knockout of either factor alone exerted minimal effects, but loss of both factors sev

SUBMITTER: Hennig AK 

PROVIDER: S-EPMC3724885 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature