Validated near-atomic resolution structure of bacteriophage epsilon15 derived from cryo-EM and modeling.

ABSTRACT: High-resolution structures of viruses have made important contributions to modern structural biology. Bacteriophages, the most diverse and abundant organisms on earth, replicate and infect all bacteria and archaea, making them excellent potential alternatives to antibiotics and therapies for multidrug-resistant bacteria. Here, we improved upon our previous electron cryomicroscopy structure of Salmonella bacteriophage epsilon15, achieving a resolution sufficient to determine the tertiary structures of both gp7 and gp10 protein subunits that form the T = 7 icosahedral lattice. This study utilizes recently established best practice for near-atomic to high-resolution (3-5 Å) electron cryomicroscopy data evaluation. The resolution and reliability of the density map were cross-validated by multi

SUBMITTER: Baker ML 

PROVIDER: S-EPMC3725109 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature