Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.

ABSTRACT: The chromatin state of pluripotency genes has been studied extensively in embryonic stem cells (ESCs) and differentiated cells, but their potential interactions with other parts of the genome remain largely unexplored. Here, we identified a genome-wide, pluripotency-specific interaction network around the Nanog promoter by adapting circular chromosome conformation capture sequencing. This network was rearranged during differentiation and restored in induced pluripotent stem cells. A large fraction of Nanog-interacting loci were bound by Mediator or cohesin in pluripotent cells. Depletion of these proteins from ESCs resulted in a disruption of contacts and the acquisition of a differentiation-specific interaction pattern prior to obvious transcriptional and phenotypic changes. Similarly, th

SUBMITTER: Apostolou E 

PROVIDER: S-EPMC3725985 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature