Identification and characterization of microRNAs in Baylisascaris schroederi of the giant panda.

ABSTRACT:

Background

Baylisascaris schroederi is one of the most significant threats to the giant panda's survival, responsible for half of the deaths reported from 2001 to 2005. MicroRNA (miRNA) has been identified as one of the key factors for gene regulations at the post-transcriptional level, and also considered as a potential control and treatment target against infectious diseases.

Methods

The present study investigated the miRNA profile of B. schroederi via high throughput sequencing and real-time quantitative PCR.

Results

A total of 18.07 million raw reads were obtained and 18.01 million were identified with high quality. By analysis of standard stem-loop structures, 108 miRNA candidates were predicted, including 60 known miRNAs and 48 novel ones. Target prediction reve

SUBMITTER: Zhao GH 

PROVIDER: S-EPMC3726319 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature