Product inhibition of cellulases studied with 14C-labeled cellulose substrates.

ABSTRACT:

Background

As a green alternative for the production of transportation fuels, the enzymatic hydrolysis of lignocellulose and subsequent fermentation to ethanol are being intensively researched. To be economically feasible, the hydrolysis of lignocellulose must be conducted at a high concentration of solids, which results in high concentrations of hydrolysis end-products, cellobiose and glucose, making the relief of product inhibition of cellulases a major challenge in the process. However, little quantitative information on the product inhibition of individual cellulases acting on cellulose substrates is available because it is experimentally difficult to assess the hydrolysis of the heterogeneous polymeric substrate in the high background of added products.

Results

The cell

SUBMITTER: Teugjas H 

PROVIDER: S-EPMC3726336 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature