Diverse activation of microglia by chemokine (C-C motif) ligand 2 overexpression in brain.

ABSTRACT:

Background

The chemokine (C-C motif) ligand 2 (CCL2) is a monocyte chemoattractant protein that mediates macrophage recruitment and migration during peripheral and central nervous system (CNS) inflammation.

Methods

To determine the impact of CCL2 in inflammation in vivo and to elucidate the CCL2-induced polarization of activated brain microglia, we delivered CCL2 into the brains of wild-type mice via recombinant adeno-associated virus serotype 9 (rAAV-9) driven by the chicken β-actin promoter. We measured microglial activation using histological and chemical measurement and recruitment of monocytes using histology and flow cytometry.

Results

The overexpression of CCL2 in the CNS induced significant activation of brain resident microglia. CD45 and major histocompatibil

SUBMITTER: Selenica ML 

PROVIDER: S-EPMC3726363 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature