Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish.

ABSTRACT:

Background

NEUROG3 is a key regulator of pancreatic endocrine cell differentiation in mouse, essential for the generation of all mature hormone producing cells. It is repressed by Notch signaling that prevents pancreatic cell differentiation by maintaining precursors in an undifferentiated state.

Results

We show that, in zebrafish, neurog3 is not expressed in the pancreas and null neurog3 mutant embryos do not display any apparent endocrine defects. The control of endocrine cell fate is instead fulfilled by two basic helix-loop-helix factors, Ascl1b and Neurod1, that are both repressed by Notch signaling. ascl1b is transiently expressed in the mid-trunk endoderm just after gastrulation and is required for the generation of the first pancreatic endocrine precursor cells. Neur

SUBMITTER: Flasse LC 

PROVIDER: S-EPMC3726459 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature