Macromolecular crowding as a suppressor of human IAPP fibril formation and cytotoxicity.

ABSTRACT: The biological cell is known to exhibit a highly crowded milieu, which significantly influences protein aggregation and association processes. As several cell degenerative diseases are related to the self-association and fibrillation of amyloidogenic peptides, understanding of the impact of macromolecular crowding on these processes is of high biomedical importance. It is further of particular relevance as most in vitro studies on amyloid aggregation have been performed in diluted solution which does not reflect the complexity of their cellular surrounding. The study presented here focuses on the self-association of the type-2 diabetes mellitus related human islet amyloid polypeptide (hIAPP) in various crowded environments including network-forming macromolecular crowding reagents and prot

SUBMITTER: Seeliger J 

PROVIDER: S-EPMC3726762 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature