Detection of differentially methylated gene promoters in failing and nonfailing human left ventricle myocardium using computation analysis.

ABSTRACT: Human dilated cardiomyopathy (DCM) is characterized by congestive heart failure and altered myocardial gene expression. Epigenetic changes, including DNA methylation, are implicated in the development of DCM but have not been studied extensively. Clinical human DCM and nonfailing control left ventricle samples were individually analyzed for DNA methylation and expressional changes. Expression microarrays were used to identify 393 overexpressed and 349 underexpressed genes in DCM (GEO accession number: GSE43435). Gene promoter microarrays were utilized for DNA methylation analysis, and the resulting data were analyzed by two different computational methods. In the first method, we utilized subtractive analysis of DNA methylation peak data to identify 158 gene promoters exhibiting DNA methyl

SUBMITTER: Koczor CA 

PROVIDER: S-EPMC3727018 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature