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A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme.

ABSTRACT: The histone variant H2A.Z plays key roles in gene expression, DNA repair, and centromere function. H2A.Z deposition is controlled by SWR-C chromatin remodeling enzymes that catalyze the nucleosomal exchange of canonical H2A with H2A.Z. Here we report that acetylation of histone H3 on lysine 56 (H3-K56Ac) alters the substrate specificity of SWR-C, leading to promiscuous dimer exchange in which either H2A.Z or H2A can be exchanged from nucleosomes. This result was confirmed in vivo, where genome-wide analysis demonstrated widespread decreases in H2A.Z levels in yeast mutants with hyperacetylated H3K56. Our work also suggests that a conserved SWR-C subunit may function as a "lock" that prevents removal of H2A.Z from nucleosomes. Our study identifies a histone modification that regulates a chromatin remodeling reaction and provides insights into how histone variants and nucleosome turnover can be controlled by chromatin regulators.

SUBMITTER: Watanabe S

PROVIDER: S-EPMC3727404 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme.

Watanabe Shinya S Radman-Livaja Marta M Rando Oliver J OJ Peterson Craig L CL

Science (New York, N.Y.) 20130401 6129

The histone variant H2A.Z plays key roles in gene expression, DNA repair, and centromere function. H2A.Z deposition is controlled by SWR-C chromatin remodeling enzymes that catalyze the nucleosomal exchange of canonical H2A with H2A.Z. Here we report that acetylation of histone H3 on lysine 56 (H3-K56Ac) alters the substrate specificity of SWR-C, leading to promiscuous dimer exchange in which either H2A.Z or H2A can be exchanged from nucleosomes. This result was confirmed in vivo, where genome-w ...[more]

PMID: 23580526

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A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme

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A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme

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