A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast.

ABSTRACT: Protein localization plays a central role in cell biology. Although powerful tools exist to assay the spatial and temporal dynamics of proteins in living cells, our ability to control these dynamics has been much more limited. We previously used the phytochrome B- phytochrome-interacting factor light-gated dimerization system to recruit proteins to the plasma membrane, enabling us to control the activation of intracellular signals in mammalian cells. Here we extend this approach to achieve rapid, reversible, and titratable control of protein localization for eight different organelles/positions in budding yeast. By tagging genes at the endogenous locus, we can recruit proteins to or away from their normal sites of action. This system provides a general strategy for dynamically activating o

SUBMITTER: Yang X 

PROVIDER: S-EPMC3727934 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature