ABCC6 is a basolateral plasma membrane protein.

ABSTRACT:

Rationale

ABCC6 plays a crucial role in ectopic calcification; mutations of the gene cause pseudoxanthoma elasticum and general arterial calcification of infancy. To elucidate the role of ABCC6 in cellular physiology and disease, it is crucial to establish the exact subcellular localization of the native ABCC6 protein.

Objective

In a recent article in Circulation Research, ABCC6 was reported to localize to the mitochondria-associated membrane and not the plasma membrane. As the suggested mitochondrial localization is inconsistent with published data and the presumed role of ABCC6, we performed experiments to determine the cellular localization of ABCC6 in its physiological environment.

Methods and results

We performed immunofluorescent labeling of frozen mouse and hum

SUBMITTER: Pomozi V 

PROVIDER: S-EPMC3727999 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature