Mapping differentiation under mixed culture conditions reveals a tunable continuum of T cell fates.

ABSTRACT: Cell differentiation is typically directed by external signals that drive opposing regulatory pathways. Studying differentiation under polarizing conditions, with only one input signal provided, is limited in its ability to resolve the logic of interactions between opposing pathways. Dissection of this logic can be facilitated by mapping the system's response to mixtures of input signals, which are expected to occur in vivo, where cells are simultaneously exposed to various signals with potentially opposing effects. Here, we systematically map the response of naïve T cells to mixtures of signals driving differentiation into the Th1 and Th2 lineages. We characterize cell state at the single cell level by measuring levels of the two lineage-specific transcription factors (T-bet and GATA3) an

SUBMITTER: Antebi YE 

PROVIDER: S-EPMC3728017 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature