Metabolic engineering of Bacillus subtilis for growth on overflow metabolites.

ABSTRACT:

Background

The genome of the important industrial host Bacillus subtilis does not encode the glyoxylate shunt, which is necessary to utilize overflow metabolites, like acetate or acetoin, as carbon source. In this study, the operon encoding the isocitrate lyase (aceB) and malate synthase (aceA) from Bacillus licheniformis was transferred into the chromosome of B. subtilis. The resulting strain was examined in respect to growth characteristics and qualities as an expression host.

Results

Our results show that the modified B. subtilis strain is able to grow on the C2 compound acetate. A combined transcript, protein and metabolite analysis indicated a functional expression of the native glyoxylate shunt of B. lichenifomis in B. subtilis. This metabolically engineered strain rev

SUBMITTER: Kabisch J 

PROVIDER: S-EPMC3728045 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature